Serotonin and Fluoxetine Receptors are Expressed in Enamel Organs

[Samsara]

http://www.ncbi.nlm.nih.gov/pubmed/21083617

Eur J Oral Sci. 2010 Dec;118(6):566-73. doi: 10.1111/j.1600-0722.2010.00778.x. Epub 2010 Sep 30.

Serotonin and fluoxetine receptors are expressed in enamel organs and LS8 cells and modulate gene expression in LS8 cells.

Riksen EA, Stunes AK, Kalvik A, Gustafsson BI, Snead ML, Syversen U, Lyngstadaas SP, Reseland JE.
Source
Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway.
_______________________________


Abstract

The selective serotonin re-uptake inhibitor (SSRI) fluoxetine is widely used in the treatment of depression in children and fertile women, but its effect on developing tissues has been sparsely investigated. The aim of this study was to investigate if enamel organs and ameloblast-derived cells express serotonin receptors that are affected by peripherally circulating serotonin or fluoxetine. Using RT-PCR and western blot analysis we found that enamel organs from 3-d-old mice and ameloblast-like cells (LS8 cells) express functional serotonin receptors, the rate-limiting enzyme in serotonin synthesis (Thp1), as well as the serotonin transporter (5HTT), indicating that enamel organs and ameloblasts are able to respond to serotonin and regulate serotonin availability.

Fluoxetine and serotonin enhanced the alkaline phosphatase activity in the cell culture medium from cultured LS8 cells, whereas the expression of enamelin (Enam), amelogenin (Amel), and matrix metalloproteinase-20 (MMP-20) were all significantly down-regulated. The secretion of vascular endothelial growth factor (VEGF), monocyte chemotactic protein 1 (MCP-1), and interferon-inducible protein 10 (IP-10) was also reduced compared with controls. In conclusion, enamel organs and ameloblast-like cells express functional serotonin receptors. Reduced transcription of enamel proteins and secretion of vascular factors may indicate possible adverse effects of fluoxetine on amelogenesis.

© 2010 Eur J Oral Sci.

PMID:
21083617
[PubMed - indexed for MEDLINE]

[Sheila]

This actually gives me *more* hope. I lost a lot of enamel on my teeth due to Paxil, and, conventionally, they say you can't re-enamel teeth. But if the cause is neuroreceptor downregulation, and you eventually upregulate again, then who's to say that you won't re-enamel?

This is clearly a job for this emoticon --

[Samsara]

This actually gives me *more* hope. I lost a lot of enamel on my teeth due to Paxil, and, conventionally, they say you can't re-enamel teeth. But if the cause is neuroreceptor downregulation, and you eventually upregulate again, then who's to say that you won't re-enamel?

This is clearly a job for this emoticon --

Here's hoping that we will re-enamel. Just occurred to me that a lot of tooth pain that many of us experience may likely be due to the loss of enamel.

LOL! I like the emoticon you chose.

Samsara

[Sheila]

Just occurred to me that a lot of tooth pain that many of us experience may likely be due to the loss of enamel.

Good point!

[Luc]

No doubt! Soooo many symptoms disappear post-WD that re-enamellation must be among them, too.

Seriously, those drugs have changed our bodies on so many levels it's not even funny. But the good thing is that this process is perfectly reversible. I wish it was a bit faster, but we will get there. Long live the enamel!

[Sheila]

This is someone worshipping someone who, although he has some vision problems, has great, strong teeth.

[Luc]

This is someone worshipping someone who, although he has some vision problems, has great, strong teeth.

LOL, the "vision problems" are big with that one, indeed.

[Sheila]

It's just w/d-induced pseudo-vision problems. It'll get better all by itself.

He won't even need any magnifying glass.